In this study, a single-chamber microbial fuel cell (SCMFC) was used to simultaneously remove 300 mg/l phenol and generate bio-electricity. During the tests, temperature and salt content were increased from 25 to 45 degrees C and from 0.5 to 2% (w/v), respectively. The results show that at 45 degrees C, the removal rate increased 4 and 5%, respectively; meanwhile, power density peak increased from 34 to 43 mW/m(2). Phenol removal rate increased 4% by adding 0.5% salt. The power density peak increased greatly to 45 mW/m(2) after adding 1% salt to the anolyte. Initial phenol concentration was consistent for all tests and cycles for 72 h. The study demonstrates that this method can be used to remove phenol and generate bio-electricity in different conditions.