Manganese peroxidase, MnP-Tra-48424, was purified and characterized from the white-rot fungus Trametes sp.48424. MnP-Tra-48424 was strongly resistant to metal ions such as Ni2+, Li+, Ca2+, Mn2+. MnP-Tra-48424 was also resistant to organic solvents such as propanediol, glycerol, and glycol. MnP-Tra-48424 decolorized dyes (indigo, anthraquinone, azo and triphenylmethane) and degraded different polycyclic aromatic hydrocarbons (PAHs). Indigo Carmine, Remazol Brilliant Blue R, Remazol Brilliant Violet 5R and Methyl Green were efficiently decolorized by MnP-Tra-48424. MnP-Tra-48424 also decolorized Indigo Carmine and Methyl Green combined with metal ions and organic solvents. The decolorization capability of MnP-Tra-48424 was not inhibited by selected metal ions and organic solvents. A combination of MnP-Tra-48424 and Lac-Tra-48424 improved the decolorization rate. In addition to dyes, MnP-Tra-48424 was effective at degrading individual PAHs (fluorene, fluoranthene, pyrene, phenanthrene, anthracene) and also PAHs in mixtures. (C) 2016 Elsevier B.V. All rights reserved.