This article reports on the influence of amine functionalization on enzyme free hydrogen peroxide (H2O2) sensing properties of Multiwall Carbon nanotubes (MWCNTs). Three amine groups with different molecular structure such as ethylene diamine (EDA), dimethylamine (DMA), triethyl amine (TEA), were functionalized on MWCNTs. Functionalization was confirmed by Raman, FT-IR and zeta potential measurements. The effect of scan rate, applied potential, pH and H2O2 concentration was investigated to evaluate the electrochemical behavior of functionalized MWCNTs. Chorono Amperometry (CA) monitoring for catalytic oxidation of the analyte was done at an potential of -0.26V vs. Ag/AgCl necessary to oxidize or reduce the H2O2 with minimal interference from the possible coexisting electroactive compounds. EDA-MWCNT/GCE combination shows good sensing response compared with pristine, DMA and TEA functionalized MWCNTs. The CA signals are linearly proportional to the concentration of H2O2 up to 9.9 x 10(-6) M with remarkable response time (< 5 sec). The H2O2 sensitivity for amine functionalized nanotubes is in the order of EDA (525.8 mA . mu M-1 .cm(-2)) > DMA (199.75 mA mu M-1 cm(-2)) > TEA (176.63 mA mu M-1 .cm(-2)). The results of analysis reveals that, the chemical structure and surface charge associated with amine functionalization plays an important role in electrochemical behavior of MWCNTs. (C) 2016 The Electrochemical Society.