Although the time-dependent buildup of asymmetric Fano line shapes in absorption spectra has been of great theoretical interest in the past decade, experimental verification of the predictions has been elusive. Here, we report the experimental observation of the emergence of a Fano resonance in the prototype system of helium by interrupting the autoionization process of a correlated two-electron excited state with a strong laser field. The tunable temporal gate between excitation and termination of the resonance allows us to follow the formation of a Fano line shape in time. The agreement with ab initio calculations validates our experimental time-gating technique for addressing an even broader range of topics, such as the emergence of electron correlation, the onset of electron-internuclear coupling, and quasi-particle formation.