Novel 3D printed alginate-BFP1 hybrid scaffolds for enhanced bone regeneration

Eun Young Heo; Na Re Ko; Min Soo Bae; Sang Jin Lee; Byung-Joon Choi; Jung Ho Kim; Hyung Keun Kim; Su A Park; Il Keun Kwon

Journal of Industrial and Engineering Chemistry, Vol.45, 61-67, January, 2017

DOI10.1016/j.jiec.2016.09.003 Export Citation

Novel 3D printed alginate-BFP1 hybrid scaffolds for enhanced bone regeneration

Eun Young Heo, Na Re Ko, Min Soo Bae, Sang Jin Lee, Byung-Joon Choi¹, Jung Ho Kim, Hyung Keun Kim², Su A Park^{3, †}, and Il Keun Kwon^†

Department of Dental Materials, School of Dentistry, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea
¹Department of Oral and Maxillofacial Surgery, School of Dentistry, Kyung Hee University, 26 Kyunghee-daero, Dongdaemun-gu, Seoul 02447, Republic of Korea
²Cardiovascular Convergence Research Center of Chonnam National University Hospital, 42 Jebong-ro, Dong-gu, Gwangju 61469, Republic of Korea
³Nature-Inspired Mechanical System Team, Nano Convergence & Manufacturing Systems Research Division, Korea Institute of Machinery & Materials, Daejeon 34103, Republic of Korea

E-mail:,

In this research, novel 3D printed alginate-peptide hybrid scaffolds were explored for enhanced bone regeneration. Bone formation peptide-1 (BFP1) was loaded into the scaffolds to promote bone regeneration. The alginate-BFP1 conjugates were synthesized via a facile EDC/NHS coupling reaction and then cross-linked in the presence of calcium ions to form hydrogel networks. The dose-dependent cytotoxicity of CaCl2 was evaluated to optimize the amount of Ca2+ for fabrication of scaffolds. In vitro and in vivo studies indicated that the alginate-based scaffolds provided a stable environment for the growth of human adipose-derived stem cells (hADSCs) and led to a synergistically enhanced bone regeneration.

Keywords:Tissue engineering;Hydrogel scaffold;BFP1;3D printing;Bone regeneration

[References]

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[Referenced By]

[1] Braddock M, Houston P, Campbell C, Ashcroft P, Physiology, 16, 208 (2001)

[2] Gong T, Xie J, Liao J, Zhang T, Lin S, Lin Y, Bone Res., 3, 15029 (2015)

[3] Kumar P, Vinitha B, Fathima G, J. Pharm. Bioallied Sci., 5, S125 (2013)

[4] Bonfiglio M, Jeter WS, Clin. Orthopaed. Rel. Res., 87, 19 (1972)

[5] Wei G, Ma PX, Biomaterials, 25, 4749 (2004)

[6] Yu HS, Won JE, Jin GZ, Kim HW, BioRes. Open Access, 1, 124 (2012)

[7] Aravamudhan A, Ramos DM, Nip J, Harmon MD, James R, Deng M, Laurencin CT, Yu X, Kumbar SG, J. Biomed. Nanotechnol., 9, 719 (2013)

[8] Zhang Y, Fan W, Ma Z, Wu C, Fang W, Liu G, Xiao Y, Acta Biomater., 6, 3021 (2010)

[9] Polo-Corrales L, Latorre-Esteves M, Ramirez-Vick JE, J. Nanosci. Nanotechnol., 14, 15 (2014)

[10] Langer R, Vacanti JP, Science, 260, 920 (1993)

[11] Ma PX, Zhang R, Xiao G, Franceschi R, J. Biomed. Mater. Res., 54, 284 (2001)

[12] Dhandayuthapani B, Yoshida Y, Maekawa T, Kumar DS, Int. J. Polymer Sci., 2011, 1 (2011)

[13] Sun J, Tan H, Materials, 6, 1285 (2013)

[14] Gombotz WR, Wee S, Adv. Drug Deliv. Rev., 31, 267 (1998)

[15] Noshi T, Yoshikawa T, Dohi Y, Ikeuchi M, Horiuchi K, Ichijima K, Sugimura M, Yonemasu K, Ohgushi H, Artif. Org., 25, 201 (2001)

[16] Alam MI, Asahina I, Ohmamiuda K, Takahashi K, Yokota S, Enomoto S, Biomaterials, 22, 1643 (2001)

[17] Senta H, Park H, Bergeron E, Drevelle O, Fong D, Leblanc E, Cabana F, Roux S, Grenier G, Faucheux N, Cytokine Growth Factor Rev., 20, 213 (2009)

[18] Kim HK, Kim JH, Park DS, Park KS, Kang SS, Lee JS, Jeong MH, Yoon TR, Biomaterials, 33, 7057 (2012)

[19] Li W, Zheng Y, Zhao X, Ge Y, Chen T, Liu Y, Zhou Y, PloS one, 11, e01502 (2016)

[20] Cho HJ, Perikamana SKM, Lee JH, Lee J, Lee KM, Shin CS, Shin H, ACS Appl. Mater. Interfaces, 6, 11225 (2014)

[21] Lee YJ, Lee JH, Cho HJ, Kim HK, Yoon TR, Shin H, Biomaterials, 34, 5059 (2013)

[22] Luo Z, Yang Y, Deng Y, Sun Y, Yang H, Wei S, Colloids Surf. B: Biointerfaces, 143, 243 (2016)

[23] Park SA, Lee SH, Kim WD, Macromol. Res., 19(7), 694 (2011)

[24] Lee SJ, Lee D, Yoon TR, Kim HK, Jo HH, Park JS, Lee JH, Kim WD, Kwon IK, Park SA, Acta Biomater., 40, 182 (2016)

[25] Lee SJ, Jo HH, Kwon SK, Lee JH, Kim WD, Lee JH, Kwon IK, Park SA, Macromol. Res., 24(4), 305 (2016)

[26] Augst AD, Kong HJ, Mooney DJ, Macromol. Biosci., 6, 623 (2006)

[27] Luo Z, Deng Y, Zhang R, Wang M, Bai Y, Zhao Q, Lyu Y, Wei J, Wei S, Colloids Surf. B: Biointerfaces, 131, 73 (2015)

[28] Sun Y, Deng Y, Ye Z, Liang S, Tang Z, Wei S, Colloids Surf. B: Biointerfaces, 111, 107 (2013)

[29] Reddi AH, Nat. Biotechnol., 16, 247 (1998)

[30] Wang M, Deng Y, Zhou P, Luo Z, Li Q, Xie B, Zhang X, Chen T, Pei D, Tang Z, Wei S, ACS Appl. Mater. Interfaces, 7, 4560 (2015)

[31] Xu A, Zhou L, Deng Y, Chen X, Xiong X, Deng F, Wei S, J. Mater. Chem. B, 4, 1878 (2016)