화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.46, 182-191, February, 2017
DOI10.1016/j.jiec.2016.10.029  Export Citation
A unique scaffold for bone tissue engineering: An osteogenic combination of graphene oxide-hyaluronic acid.chitosan with simvastatin
Afeesh Rajan Unnithan1, 2, †, Arathyram Ramachandra Kurup Sasikala1, 3, Chan Hee Park1, 2, †, and Cheol Sang Kim1, 2, †
  • 1Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju, Republic of Korea
  • 2Division of Mechanical Design Engineering, Chonbuk National University, Jeonju, Republic of Korea
  • 3, Korea
E-mail:, ,
The present study explores the first ever fabrication of graphene oxide (GO)-Chitosan (CS)-Hyaluronic acid (HA) based bioactive composite scaffold containing an osteogenesis-inducing drug simvastatin (SV) for bone tissue engineering application. Porosity, density, swelling, degradation and biomineralization studies favored the SV loaded GO-CS-HA scaffold in comparison to the conventional CS and HA based scaffolds. The in vitro results showed that the SV also offers a significant influence on osteogenesis and biomineralization and it possess excellent biocompatibility to be used as a bone tissue engineering scaffold, which is able to persuade osteogenesis and mineralization.
Keywords:Graphene oxide;Chitosan;Hyaluronic acid;Simvastatin;Bone tissue engineering
  1. Mohandas A, Anisha BS, Chennazhi KP, Jayakumar R, Colloids Surf. B: Biointerfaces, 127, 105 (2015)
  2. Unnithan AR, Sasikala ARK, Murugesan P, Gurusamy M, Wu DM, Park CH, Kim CS, Int. J. Biol. Macromol., 77, 1 (2015)
  3. Burg KJL, Porter S, Kellam JF, Biomaterials, 21, 2347 (2000)
  4. Kavya KC, Jayakumar R, Nair S, Chennazhi KP, Int. J. Biol. Macromol., 59, 255 (2013)
  5. GhavamiNejad A, Sasikala ARK, Unnithan AR, Thomas RG, Jeong YY, Vatankhah-Varnoosfaderani M, Stadler FJ, Park CH, Kim CS, Adv. Funct. Mater., 25(19), 2867 (2015)
  6. Depan D, Misra RDK, J. Biomed. Mater. Res. A, 100a, 3080 (2012)
  7. Hesaraki S, Nezafati N, Bioprocess. Biosyst. Eng., 37, 1507 (2014)
  8. Jaikumar D, Sajesh KM, Soumya S, Nimal TR, Chennazhi KP, Nair SV, Jayakumar R, Int. J. Biol. Macromol., 74, 318 (2015)
  9. Depan D, Surya PKCV, Girase B, Misra RDK, Acta Biomater., 7, 2163 (2011)
  10. Anitha A, Sowmya S, Kumar PTS, Deepthi S, Chennazhi KP, Ehrlich H, Tsurkan M, Jayakumar R, Prog. Polym. Sci, 39, 1644 (2014)
  11. Kumar RA, Sivashanmugam A, Deepthi S, Iseki S, Chennazhi KP, Nair SV, Jayakumar R, ACS Appl. Mater. Interfaces, 7, 9399 (2015)
  12. Li ZS, Ramay HR, Hauch KD, Xiao DM, Zhang MQ, Biomaterials, 26, 3919 (2005)
  13. Thein-Han WW, Misra RDK, Acta Biomater., 5, 1182 (2009)
  14. Muzzarelli RAA, Mattiolibelmonte M, Tietz C, Biagini R, Ferioli G, Brunelli MA, Fini M, Giardino R, Ilari P, Biagini G, Biomaterials, 15, 1075 (1994)
  15. Anisha BS, Biswas R, Chennazhi KP, Jayakumar R, Int. J. Biol. Macromol., 62, 310 (2013)
  16. Leach JB, Bivens KA, Patrick CW, Schmidt CE, Biotechnol. Bioeng., 82(5), 578 (2003)
  17. Solchaga LA, Dennis JE, Goldberg VM, Caplan AI, J. Orthop. Res., 17, 205 (1999)
  18. Kim J, Kim IS, Cho TH, Lee KB, Hwang SJ, Tae G, Noh I, Lee SH, Park Y, Sun K, Biomaterials, 28, 1830 (2007)
  19. Collins MN, Birkinshaw C, Carbohydr. Polym., 92, 1262 (2013)
  20. Quinlan E, Lopez-Noriega A, Thompson E, Kelly HM, Cryan SA, O’Brien FJ, J. Control. Release, 198, 71 (2015)
  21. Nettles DL, Elder SH, Gilbert JA, Tissue Eng., 8, 1009 (2002)
  22. Nishida E, Miyaji H, Takita H, Kanayama I, Tsuji M, Akasaka T, Sugaya T, Sakagami R, Kawanami M, Jpn. J. Appl. Phys., 53 (2014)
  23. Elkhenany H, Amelse L, Lafont A, Bourdo S, Caldwell M, Neilsen N, Dervishi E, Derek O, Biris AS, Anderson D, Dhar M, J. Appl. Toxicol., 35, 367 (2015)
  24. Xie XY, Hu KW, Fang DD, Shang LH, Tran SD, Cerruti M, Nanoscale, 7, 7992 (2015)
  25. Sayyar S, Murray E, Thompson BC, Chung J, Officer DL, Gambhir S, Spinks GM, Wallace GG, J. Mater. Chem. B, 3, 481 (2015)
  26. Pandele AM, Ionita M, Crica L, Dinescu S, Costache M, Iovu H, Carbohydr. Polym., 102, 813 (2014)
  27. Geim AK, Novoselov KS, Nat. Mater., 6(3), 183 (2007)
  28. Ionita M, Pandele MA, Iovu H, Carbohydr. Polym., 94, 339 (2013)
  29. Dreyer DR, Park S, Bielawski CW, Ruoff RS, Chem. Soc. Rev., 39, 228 (2010)
  30. Yang K, Wan JM, Zhang S, Tian B, Zhang YJ, Liu Z, Biomaterials, 33, 2206 (2012)
  31. Justin R, Chen BQ, Carbohydr. Polym., 103, 70 (2014)
  32. Yang H, Bremner HD, Tao L, Li H, Hu J, Zhu L, Carbohydr. Polym., 135, 72 (2015)
  33. Depan D, Girase B, Shah JS, Misra RDK, Acta Biomater., 7, 3432 (2011)
  34. Dinescu S, Ionita M, Pandele AM, Galateanu B, Iovu H, Ardelean A, Costache M, Hermenean A, Bio-Med. Mater. Eng., 24, 2249 (2014)
  35. Jung HS, Lee MY, Kong WH, Do IH, Hahn Sk, RSC Adv., 4, 14197 (2014)
  36. Depan D, Misra RDK, Acta Biomater., 9, 6084 (2013)
  37. Zhou YS, Ni YW, Liu YS, Zeng BJ, Xu YW, Ge WS, Biomaterials, 31, 5325 (2010)
  38. Bae MS, Yang DH, Lee JB, Heo DN, Kwon YD, Youn IC, Choi K, Hong JH, Kim GT, Choi YS, Hwang EH, Kwon IK, Biomaterials, 32, 8161 (2011)
  39. Zhang HX, Xiao GY, Wang X, Dong ZG, Ma ZY, Li L, Li YH, Pan X, Nie L, J. Biomed. Mater. Res. Part A, 103A, 3250 (2015)
  40. Yan Q, Xiao LQ, Tan L, Sun W, Wu T, Chen LW, Mei Y, Shi B, J. Biomed. Mater. Res. A, 103A, 3580 (2015)
  41. Samorezov JE, Alsberg E, Adv. Drug Deliv. Rev., 84, 45 (2015)
  42. Saravanakumar B, Mohan R, Kim SJ, Mater. Res. Bull., 48(2), 878 (2013)
  43. Kumar PTS, Ramya C, Jayakumar R, Nair SKV, Lakshmanan VK, Colloids Surf. B: Biointerfaces, 106, 109 (2013)
  44. Collins MN, Birkinshaw C, J. Appl. Polym. Sci., 120(2), 1040 (2011)
  45. Simeonova M, Ivanova G, Enchev V, Markova N, Kamburov M, Petkov C, Devery A, O’Connor R, Brougham D, Acta Biomater., 5, 2109 (2009)
  46. Kokubo T, Takadama H, Biomaterials, 27, 2907 (2006)
  47. Park SN, Park JC, Kim HO, Song MJ, Suh H, Biomaterials, 23, 1205 (2002)
  48. Anisha BS, Sankar D, Mohandas A, Chennazhi KP, Nair SV, Jayakumar R, Carbohydr. Polym., 92, 1470 (2013)
  49. Marcano DC, Kosynkin DV, Berlin JM, Sinitskii A, Sun ZZ, Slesarev A, Alemany LB, Lu W, Tour JM, ACS Nano, 4, 4806 (2010)
  50. Kumar S, Koh J, Int. J. Biol. Macromol., 70, 559 (2014)
  51. Rana VK, Choi MC, Kong JY, Kim GY, Kim MJ, Kim SH, Mishra S, Singh RP, Ha CS, Macromol. Mater. Eng., 296, 131 (2011)
  52. Deepthi S, Jeevitha K, Sundaram MN, Chennazhi KP, Jayakumar R, Chem. Eng. J., 260, 478 (2015)
  53. Ming P, Song ZF, Gong SS, Zhang YY, Duan JL, Zhang Q, Jiang L, Cheng QF, J. Mater. Chem. A, 3, 21194 (2015)
  54. Min SH, Jin HH, Park HY, Park IM, Park HC, Yoon SY, Mater. Sci. Forum, 510-511, 754 (2006)
  55. Ju J, Zheng YM, Jiang L, Acc. Chem. Res., 47, 2342 (2014)
  56. Depan D, Shah JS, Misra RDK, Polym. Degrad. Stabil., 98, 2331 (2013)
  57. Alsberg E, Kong HJ, Hirano Y, Smith MK, Albeiruti A, Mooney DJ, J. Dent. Res., 82, 903 (2003)
  58. Valachova MBK, Topolsk D, Sasinkova V, Juranek I, Collins MN, Soltes L, Carbohydr. Polym., 134, 516 (2015)
  59. Wan SJ, Li YC, Peng JS, Hu H, Cheng QF, Jiang L, ACS Nano, 9, 708 (2015)
  60. Wang JF, Cheng QF, Lin L, Jiang L, ACS Nano, 8, 2739 (2014)
  61. Gong SS, Cui W, Zhang Q, Cao AY, Jiang L, Cheng QF, ACS Nano, 9, 11568 (2015)
  62. Cheng QF, Duan JL, Zhang Q, Jiang L, ACS Nano, 9, 2231 (2015)
  63. Wan SJ, Peng JS, Li YC, Hu H, Jiang L, Cheng QF, ACS Nano, 9, 9830 (2015)
  64. Cui W, Li MZ, Liu JY, Wang B, Zhang C, Jiang L, Cheng QF, ACS Nano, 8, 9511 (2014)
  65. Cheng QF, Wu MX, Li MZ, Jiang L, Tang ZY, Angew. Chem.-Int. Edit., 52, 3750 (2013)
  66. Depan D, Pesacreta TC, Misra RDK, Biomater. Sci. UK, 2, 264 (2014)
  67. Madhumathi K, Shalumon KT, Rani VVD, Tamura H, Furuike T, Selvamurugan N, Nair SV, Jayakumar R, Int. J. Biol. Macromol., 45, 12 (2009)
  68. Kretlow JD, Mikos AG, Tissue Eng., 13, 927 (2007)