화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.47, 62-73, March, 2017
DOI10.1016/j.jiec.2016.11.015  Export Citation
Silver nanoparticles suppresses brain-derived neurotrophic factor-induced cell survival in the human neuroblastoma cell line SH-SY5Y
Jung Hyun Park, Sangiliyandi Gurunathan, Yun-Jung Choi, Jae Woong Han, Hyuk Song, and Jin-Hoi Kim†
  • Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea
E-mail:
Silver nanoparticles (AgNPs) are used as cytotoxic agents in various cancer cell lines; however, the cytotoxic effects of AgNPs on human neuroblastoma cells remain poorly understood. Here we report the mechanisms of action of AgNPs on brain-derived neurotrophic factor (BDNF) activation in human neuroblastoma cells using AgNPs. The results from biochemical, cellular and western blot analysis indicated that AgNPs significantly inhibit BDNF induced cell survival by enhanced leakage of lactate dehydrogenase, activation of reactive oxygen species, DNA damage, and modulation of various signaling molecules. Thus, AgNPs could serve as a potential candidate for developing novel therapeutic molecules for cancers.
Keywords:Silver nanoparticles;Brain-derived neurotrophic factor;Cell viability;Apoptosis;Cellular signaling
  1. Gurunathan S, Han JW, Kwon DN, Kim JH, Nanoscale Res. Lett., 9 (2014)
  2. Gurunathan S, Lee KJ, Kalishwaralal K, Sheikpranbabu S, Vaidyanathan R, Eom SH, Biomaterials, 30, 6341 (2009)
  3. Gurunathan S, Raman J, Malek NA, John PA, Vikineswary S, Int. J. Nanomedicine, 8, 4399 (2013)
  4. Nadworny PL, Landry BK, Wang J, Tredget EE, Burrell RE, Wound Repair Regen., 18, 254 (2010)
  5. Sriram MI, Kanth SBM, Kalishwaralal K, Gurunathan S, Int. J. Nanomedicine, 5, 753 (2010)
  6. Deepak V, Umamaheshwaran PS, Guhan K, Nanthini RA, Krithiga B, Jaithoon NMH, Gurunathan S, Colloids Surf. B: Biointerfaces, 86, 353 (2011)
  7. Tran TTT, Vu TTH, Nguyen TH, Mater. Lett., 105, 220 (2013)
  8. Gurunathan S, Jeong JK, Han JW, Zhang XF, Park JH, Kim JH, Nanoscale Res. Lett., 10, 1 (2015)
  9. Gurunathan S, Kalishwaralal K, Vaidyanathan R, Deepak V, Pandian SRK, Muniyandi J, Hariharan N, Eom SH, Colloids Surf. B: Biointerfaces, 74, 328 (2009)
  10. Emerich DF, Thanos CG, Biomol. Eng., 23, 171 (2006)
  11. Choi BH, Lee HH, Jin SM, Chun SK, Kim SH, Nanotechnology, 18 (2007)
  12. Mandal S, Selvakannan PR, Roy D, Chaudhari RV, Sastry M, Chem. Commun., 3002 (2002)
  13. Gref R, Couvreur P, Barratt G, Mysiakine E, Biomaterials, 24, 4529 (2003)
  14. Neouze MA, Schubert U, Monatsh. Chem., 139, 183 (2008)
  15. Ravindran A, Singh A, Raichur AM, Chandrasekaran N, Mukherjee A, Colloids Surf. B: Biointerfaces, 76, 32 (2010)
  16. Ravindran A, Chandran P, Khan SS, Colloids Surf. B: Biointerfaces, 105, 342 (2013)
  17. Thakkar KN, Mhatre SS, Parikh RY, Nanomedicine, 6, 257 (2010)
  18. Mandal S, Selvakannan P, Phadtare S, Pasricha R, Sastry M, Proc. Indian Acad. Sci. Chem. Sci., 114, 513 (2002)
  19. Safavi A, Zeinali S, Yazdani M, Amino Acids, 43, 1323 (2012)
  20. Hussain SM, Hess KL, Gearhart JM, Geiss KT, Schlager JJ, Toxicol. in Vitro, 19, 975 (2005)
  21. Hussain SM, Javorina AK, Schrand AM, Duhart HM, Ali SF, Schlager JJ, Toxicol. Sci., 92, 456 (2006)
  22. Braydich-Stolle L, Hussain S, Schlager JJ, Hofmann MC, Toxicol. Sci., 88, 412 (2005)
  23. Zhang XF, Gurunathan S, Kim JH, Int. J. Nanomedicine, 10, 6243 (2015)
  24. Gurunathan S, Han JW, Dayem AA, Eppakayala V, Park JH, Cho SG, Lee KJ, Kim JH, J. Ind. Eng. Chem., 19(5), 1600 (2013)
  25. Gurunathan S, Han JW, Park JH, Kim E, Choi YJ, Kwon DN, Kim JH, Int. J. Nanomedicine, 10, 6257 (2015)
  26. AshaRani PV, Mun GLK, Hande MP, Valiyaveettil S, ACS Nano, 3, 279 (2009)
  27. Foldbjerg R, Dang DA, Autrup H, Arch. Toxicol., 85, 743 (2011)
  28. Gurunathan S, Han JW, Eppakayala V, Jeyaraj M, Kim JH, Biomed. Res Int., doi:http://dx.doi.org/10.1155/2013/535796 Article ID 535796., 10 (2013)
  29. Nallathamby PD, Xu XHN, Nanoscale, 2, 942 (2010)
  30. Sanpui P, Chattopadhyay A, Ghosh SS, ACS Appl. Mater. Interfaces, 3, 218 (2011)
  31. Liu JH, Zhao YX, Guo QQ, Wang Z, Wang HY, Yang YX, Huang YZ, Biomaterials, 33, 6155 (2012)
  32. Xu R, Ma J, Sun XC, Chen ZP, Jiang XL, Guo ZR, Huang L, Li Y, Wang M, Wang CL, Liu JW, Fan X, Gu JY, Chen X, Zhang Y, Gu N, Cell Res., 19, 1031 (2009)
  33. Lin J, Huang ZH, Wu H, Zhou W, Jin PP, Wei PF, Zhang YJ, Zheng F, Zhang JQ, Xu J, Hu Y, Wang YH, Li YJ, Gu N, Wen LP, Autophagy, 10, 2006 (2014)
  34. Tivnan A, Orr WS, Gubala V, Nooney R, Williams DE, McDonagh C, Prenter S, Harvey H, Domingo-Fernandez R, Bray IM, Piskareva O, Ng CY, Lode HN, Davidoff AM, Stallings RL, PLoS One, 7 (2012)
  35. Wagner LM, Danks MK, J. Cell. Biochem., 107, 46 (2009)
  36. Schor NF, J. Neurooncol., 41, 159 (1999)
  37. Xie HR, Hu LS, Li GY, Chin. Med. J. Peking, 123, 1086 (2010)
  38. Singh J, Kaur G, Brain Res., 1154, 8 (2007)
  39. Alberio T, Lopiano L, Fasano M, FEBS J., 279, 1146 (2012)
  40. Han JW, Gurunathan S, Jeong JK, Choi YJ, Kwon DN, Park JK, Kim JH, Nanoscale Res. Lett., 9 (2014)
  41. Chen HC, Vermulst M, Wang YE, Chomyn A, Prolla TA, McCaffery JM, Chan DC, Cell, 141, 280 (2010)
  42. Henglein A, J. Phys. Chem., 97, 5457 (1993)
  43. Sastry CS, Srinivas Y, Rao PV, Talanta, 44, 517 (1997)
  44. Sastry M, Patil V, Sainkar SR, J. Phys. Chem. B, 1012, 1404 (1998)
  45. Zhang XF, Liu ZG, Shen W, Gurunathan S, Int. J. Mol. Sci., 17, 1534 (2016)
  46. Mu QS, Hondow NS, Krzeminski L, Brown AP, Jeuken LJC, Routledge MN, Part. Fibre Toxicol., 9 (2012)
  47. Schaerer E, Neutra MR, Kraehenbuhl JP, J. Membr. Biol., 123, 93 (1991)
  48. Geiser M, Rothen-Rutishauser B, Kapp N, Schurch S, Kreyling W, Schulz H, Semmler M, Hof VI, Heyder J, Gehr P, Environ. Health Perspect., 113, 1555 (2005)
  49. Rothen-Rutishauser BM, Schurch S, Haenni B, Kapp N, Gehr P, Environ. Sci. Technol., 40, 4353 (2006)
  50. Banerji SK, Hayes MA, Langmuir, 23(6), 3305 (2007)
  51. Takac T, Pechan T, Samajova O, Samaj J, J. Proteome Res., 12, 4435 (2013)
  52. Vranic S, Boggetto N, Contremoulins V, Mornet S, Reinhardt N, Marano F, Baeza-Squiban A, Boland S, Part. Fibre Toxicol., 10, 2 (2013)
  53. McIntosh DP, Tan XY, Oh P, Schnitzer JE, Proc. Natl. Acad. Sci. U. S. A., 99, 19996 (2002)
  54. Ivanov AI, Pharmacological Inhibition of Endocytic Pathways: Is it Specific Enough to be Useful? in: A.I. Ivanov (Ed.), Humana Press, Totowa, NJ, 2008 p.15.
  55. Hardwick JP, Gene Cell Tissue, 1, e21227 (2014)
  56. Yoisungnern T, Choi YJ, Han JW, Kang MH, Das J, Gurunathan S, Kwon DN, Cho SG, Park C, Chang WK, Chang BS, Parnpai R, Kim JH, Sci. Rep. UK, 5 (2015)
  57. Scarpulla RC, J. Bioenerg. Biomembr., 29, 109 (1997)
  58. Kocher HM, Senkus R, Al-Nawab M, Hendry BM, Nephrol. Dial. Transpl., 20, 886 (2005)
  59. Rebollo A, Perez-Sala D, Martinez C, Oncogene, 18, 4930 (1999)
  60. Trinei M, Berniakovich I, Pelicci PG, Giorgio M, BBA Bioenerg., 1757, 624 (2006)
  61. Salvioli S, Bonafe M, Capri M, Monti D, Franceschi C, Febs Lett., 492, 9 (2001)
  62. Park J, Lee J, Choi C, PLoS One, 6 (2011)
  63. Pylayeva-Gupta Y, Grabocka E, Bar-Sagi D, Nat. Rev. Cancer, 11, 761 (2011)
  64. Heeren G, Jarolim S, Laun P, Rinnerthaler M, Stolze K, Perrone GG, Kohlwein SD, Nohl H, Dawes IW, Breitenbach M, FEMS Yeast Res., 5, 157 (2004)
  65. Longo VD, SAGE KE, 2004, pe36 (2004)
  66. Hu YM, Lu WQ, Chen G, Wang P, Chen Z, Zhou Y, Ogasawara M, Trachootham D, Feng L, Pelicano H, Chiao PJ, Keating MJ, Garcia-Manero G, Huang P, Cell Res., 22, 399 (2012)
  67. Kim A, Davis R, Higuchi M, Cell Death Dis., 6, e1825 (2015)
  68. Kaplan DR, Matsumoto K, Lucarelli E, Thiele CJ, Neuron, 11, 321 (1993)
  69. Coccini T, Manzo L, Bellotti V, De Simone U, ScientificWorldJournal, 2014, 259765 (2014)
  70. Sohaebuddin SK, Thevenot PT, Baker D, Eaton JW, Tang LP, Part. Fibre Toxicol., 7 (2010)
  71. Wilson MR, Lightbody JH, Donaldson K, Sales J, Stone V, Toxicol. Appl. Pharmacol., 184, 172 (2002)
  72. Kim SH, Ko JW, Koh SK, Lee IC, Son JM, Moon C, Kim SH, Shin DH, Kim JC, Mol. Cell. Toxicol., 10, 173 (2014)
  73. Liu F, Mahmood M, Xu Y, Watanabe F, Biris AS, Hansen DK, Inselman A, Casciano D, Patterson TA, Paule MG, Slikker W, Wang C, Front. Neurosci. Switz, 9 (2015)
  74. Dayem AA, Kim B, Gurunathan S, Choi HY, Yang G, Saha SK, Han D, Han J, Kim K, Kim JH, Cho SG, Biotechnol. J., 9, 934 (2014)
  75. Grecco HE, Schmick M, Bastiaens PIH, Cell, 144, 897 (2011)
  76. Rauch J, Kolch W, Mahmoudi M, Sci. Rep. UK, 2, 868 (2012)
  77. Marano F, Hussain S, Rodrigues-Lima F, Baeza-Squiban A, Boland S, Arch. Toxicol., 85, 733 (2011)
  78. Castiglioni S, Cazzaniga A, Perrotta C, Maier JAM, Toxicol. Lett., 237, 237 (2015)
  79. Mebratu Y, Tesfaigzi Y, Cell Cycle, 8, 1168 (2009)
  80. Subramaniam S, Unsicker K, FEBS J., 277, 22 (2010)
  81. Gurunathan S, Park JH, Han JW, Kim JH, Int. J. Nanomedicine, 10, 4203 (2015)
  82. Das S, Tosaki A, Bagchi D, Maulik N, Das DK, J. Pharmacol. Exp. Ther., 314, 762 (2005)
  83. Comfort KK, Maurer EI, Braydich-Stolle LK, Hussain SM, ACS Nano, 5, 10000 (2011)
  84. Han JW, Jeong JK, Gurunathan S, Choi YJ, Das J, Kwon DN, Cho SG, Park C, Seo HG, Park JK, Kim JH, Nanotoxicology, 10, 361 (2016)
  85. Cardaci S, Filomeni G, Rotilio G, Ciriolo MR, Mol. Pharmacol., 74, 1234 (2008)
  86. Chowdhury R, Chowdhury S, Roychoudhury P, Mandal C, Chaudhuri K, Apoptosis, 14, 108 (2009)
  87. Ahamed M, Karns M, Goodson M, Rowe J, Hussain SM, Schlager JJ, Hong Y, Toxicol. Appl. Pharmacol., 233, 404 (2008)