Simultaneous saccharification and fermentation of alkali-pretreated corncob under optimized conditions using cold-tolerant indigenous holocellulase

Anamika Sharma; Vikrant Nain; Rameshwar Tiwari; Surender Singh; Anurup Adak; Pawan Kumar Singh Nain; Lata Nain

Korean Journal of Chemical Engineering, Vol.34, No.3, 773-780, March, 2017

DOI10.1007/s11814-016-0334-9 Export Citation

Simultaneous saccharification and fermentation of alkali-pretreated corncob under optimized conditions using cold-tolerant indigenous holocellulase

Anamika Sharma^{1, 2}, Vikrant Nain², Rameshwar Tiwari^{1, 3}, Surender Singh^{1, 3}, Anurup Adak^{1, 3}, Pawan Kumar Singh Nain⁴, and Lata Nain^{1, 3, †}

¹Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India
²School of Biotechnology, Gautam Buddha University, Greater Noida, Uttar Pradesh-201312 India
³, India
⁴Design and Mechatronic Division, School of Civil and Mechanical Engineering, Galgotias University, Uttar Pradesh-201312, India

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The present investigation was aimed towards pretreatment optimization of corncob to maximize cellulose and hemicellulose recovery, followed by substrate selection for holocellulase production using psychrotolerant Aspergillus niger SH3. Dilute alkali pretreatment (1.5% NaOH) resulted in higher recovery of cellulose (59.66%) and hemicellulose (28.34%) from corncob, while corn stover proved to be the best substrate for holocellulase production. Further, saccharification was optimized by Box-Behnken design to select the suitable conditions for maximum sugar release from pretreated corncob. The optimum conditions for maximum sugar release were 8% (w/v) substrate loading, 11 FPU/gds enzyme loading at temperature 38 °C and pH 3.0 which resulted in 114.5% higher sugar yield (912mg/gds of pretreated biomass) as compared with un-optimized conditions (425.35mg/gds). Theoretical yield of 48.8% ethanol was achieved through simultaneous saccharification and fermentation (SSF) using pretreated corncob. This study illustrates the potential of different corn residues as a promising substrate for bioethanol production.

Keywords:Bioethanol;Corn residues;Aspergillus niger;Saccharification;Fermentation;Box-Behnken

[References]

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[1] Segal L, Creely J, Martin A, Conrad C, Text. Res. J., 29, 786 (1959)

[2] Bahcegul E, Toraman HE, Erdemir D, Akinalan B, Ozkan N, Bakir U, RSC Adv., 4(64) (2014)

[3] Fan X, Cheng G, Zhang H, Li M, Wang S, Yuan Q, Carbohydr. Polym., 114, 21 (2014)

[4] Kumar AK, Parikh BS, Bioresour. Bioprocess, 2, 1 (2015)

[5] Li CL, Knierim B, Manisseri C, Arora R, Scheller HV, Auer M, Vogel KP, Simmons BA, Singh S, Bioresour. Technol., 101(13), 4900 (2010)

[6] Kljun A, Benians TAS, Goubet F, Meulewaeter F, Knox JP, Blackburn RS, Biomacromolecules, 12(11), 4121 (2011)

[7] Pandiyan K, Tiwari R, Rana S, Arora A, Singh S, Saxena AK, Nain L, World J. Microbiol. Biotechnol., 30, 55 (2014)

[8] Moharram MA, Mahmoud OM, J. Appl. Polym. Sci., 105(5), 2978 (2007)

[9] Oh SY, Yoo DI, Shin Y, Kim HC, Kim HY, Chung YS, Park WH, Youk JH, Carbohydr. Res., 340, 2376 (2005)