The use of biomass for energy production is one of the promising alternatives identified by the European Union to reduce greenhouse gas emissions and to ensure energy supply security. The aim of this research is to analyse the potential energy production from pruning residues of vineyards and olive groves in four municipalities of the Umbria Region, along with its economic and environmental sustainability. Geospatial, economic and environmental analyses were carried out. The integrated approach was used to estimate the total quantity of biomass produced in that area and to identify a logistic network for its transportation. Assuming a power plant whose size and technical properties were based on the analysis results, chain costs and potential profit margins were also calculated and resulting CO2 emissions estimated. The results show that the fragmentation and disprsion of agricultural activities, as well as technical and logistical decisions noticeably affect the economic and environmental sustainability of the proposed agro-energetic chain, in terms of both energy balance and CO2 emissions. (C) 2016 Elsevier Ltd. All rights reserved.