A facile and cost-effective modification of screen-printed carbon electrode employing a graphene oxide-poly(acrylic acid) film allows both good electrochemical reactivity and electrode surface functionalization with antibody. The analytical performances of the modified electrode for detection of human immunoglobulin G (IgG) were satisfied with a linear concentration range of 1.0-100 ng mL(-1), sensitivity of 1.2318 mu A cm(-2) mL ng(-1), and a limit of detection of 0.54 ng mL(-1). The proposed electrode is promising in sensitive and selective clinical analysis to detect IgG at physiological concentration.