Print mottle is a print defect that occurs more or less on prints and has a negative influence on the evenness in print. Several methods to assess print mottle have been proposed. In this article, we analyzed three methods for print mottle evaluation: Histogram Mottle Macro, Gray level co-occurrence matrix (GLCM) and M-score. The aim was to compare them and to determine their relation to perceived non-uniformity obtained by visual assessment. In the experimental part, we printed four different substrates with different optical properties with digital printing machine based on electrophotographic process. The surface of papers was characterized by measuring optical paper properties. The results obtained in this study suggest that GLCM method should be used for print mottle assessment. This method has the strongest correlation with the visual experience of print mottle. (C) 2015 Wiley Periodicals, Inc. Col Res Appl, 41, 493-499, 2016; Published Online 2 september 2015 in Wiley Online Library (wileyonlinelibrary.com).