In the East of Algeria, the city of Constantine called for its water supply, the Hamma groundwater resource. The high content of hydrogen carbonate and calcium justify the very important scaling power, it contains 82 % by mass of these ions. This paper focuses on the application of a new green inhibitor "RS1600" for reducing calcium carbonate scale formation. RS1600 is a type of water soluble vitamin, it has proven to be green scale inhibitor given his nontoxic and biodegradable features. The inhibiting effect occurs at very low concentration. Its performance is evaluated and compared using different chemical and electrochemical anti-scaling methods. The nucleation and scaling time were identified. The ratio between homogeneous and heterogeneous nucleation was determined. IR, X-ray diffraction and Raman spectroscopy analyses demonstrated modifications in crystalline structure, the CaCO3 crystallize from calcite to vaterite. The study suggested that RS1600 is an excellent green chemical. (C) 2016 Elsevier B.V. All rights reserved.