Jatropha-oil based polyurethane is one of the initiative for replacing conventional petroleum based polyurethane. The vegetable oil-based polyurethane is more cost-effective and synthesize from renewable resources. Polyurethane was synthesized through prepolymerization method between jatropha oil-based polypl and diphenylmethane 4, 4'diisocyanate, (MDI) in inert condition. Then, lithium perchloride ion (LiCIO4) was added to the polyurethane system to form electrolyte film via solution casting technique. The polymer electrolytes were prepared by varying the amount of LiCIO4 ion 10 wt.% to 30 wt. %. The highest conductivity is achieved at 25 wt.% of LiCIO4 salt content, which is 1.29 x 10(-4)S/cm at room temperature 30 degrees C. The FTIR results showed the shifting of carbonyl group (C=O) (1750 cm(-1)-1730 cm(-1)), ether and ester group (C-O-C) (1300 cm(-1)-1000 cm(-1)) and amine functional groups (N-H) (1650 cm(-1)-1500 cm(-1)) in polyurethane electrolytes from the blank polyurethane shows that oxygen and nitrogen atom acts as electron donor in the electrolytes system. It also confirmed that the intermolecular reaction had occurred in the electrolytes, system. While, the XRD analysis showed the semi-crystalline properties of polyurethane have been reduced to amorphous phase upon the increasing addition of lithium ion. SEM results revealed the morphology analysis of the polyurethane electrolytes. There is homogenous and smooth surface in polyurethane and the dissociation of salt was observed after the addition of salt indicates there was interaction between salt and the polymer host. (C) 2016 Elsevier Ltd. All rights reserved.