An ultrathin NiCo layered double hydroxide (LDH) nanosheet array was constructed on carbon cloth (CC) by one-pot co-precipitation approach. The NiCo LDH nanosheets possess high surface specific area and their interconnected three-dimensional (3D) structure allows easy access for electrolyte ions to process rapid and reversible faradic reactions. The as-prepared NiCo LDH/CC composites show a remarkable activity for glucose oxidation, which can be used as a binder-free biosensor for glucose detection. Under optimized conditions, the sensitivity can reach up to 5.12 mu A mu M-1 cm(-2), the liner range covers four orders of magnitude from 1 mu M to 1.5 mM, and the detection limit can be low as 0.12 mu M. Besides, this biosensor also shows superior selectivity, good stability, and easy reproducibility, holding promise as a good candidate for enzymeless glucose sensing. (C) 2016 Elsevier Ltd. All rights reserved.