This paper presents the results of a study on a diesel fuel containing a biocomponent obtained by the innovative ZEOFINING process. In contrast to a typical hydrotreated vegetable oil (HVO) biocomponent, the biocomponent from the ZEOFINING process contains paraffin naphthenes and aromatics as well as n-paraffins and isoparaffins. The composition and characteristics of this compound differ essentially from those of other biocomponents, in that are typically used for diesel oil production. The biocomponent obtained in the ZEOFINING process was characterized by parameters such as cetane number, density, sulfur content, carbon residue, ash residue, flash point, and low-temperature properties that are consistent with the requirements of the EN 590/standard. The use of zeo-HVO can significantly increase the content of biocomponents in diesel fuel, as shown by the example of the ZHV70 fuel with 70 vol % content of biocomponents. The properties of this fuel comply with the requirements of the EN 590 standard.