This work reports a comparative study on the preparation and characterization of a novel carbon nanotube (CNT) based stretchable electrode aiming at developing wearable electrical biosensors. Polydimethylsiloxane (PDMS) was chosen as the elastomeric substrate for electrodes due to its promising biocompatibility, remarkable mechanical properties and ease of use. A new structure of the electrode composited by three layers has been proposed which showed significant improvement in electrochemical and mechanical properties. A simple solution-based fabrication method was employed to make CNT (active material) coated PDMS/CNT composite thin film which was integrated with a pre-cured PDMS film substrate. The electrochemical activity of the electrodes composed by single-wall carbon nanotube (SWCNT) and multi-wall carbon nanotube (MWCNT) was studied and compared by cyclic-voltammetry test. Two electrodes with surface concentration of 0.5 mg/cm(2) SWCNT and 0.75 mg/cm(2) MWCNT showed optimized electrochemical redox signals of K-3[Fe(CN)(6)] in PBS buffer solution at pH 7.4. The resistance changes of the stretchable electrodes at different stretch levels were characterized using uniaxial stretcher machine. The result showed that in the specific surface concentration of 0.5 mg/cm(2) SWCNT and thickness of 500 mu m, the electrode can maintain its surface resistance as low as 50 Omega/cm up to 300% stretch. The novel CNT/PDMS Composed stretchable electrode has great potential for the development of flexible soft bioelectronics such as wearable biosensors. (C) 2016 Elsevier B.V. All rights reserved.