The objective of this research was to evaluate interactions of hydrogen and hydroxide ions with bilayer membranes formed from phosphatidylcholine and lysophosphatidylcholine. Theoretical deliberations were successfully verified by experimental research studies that were conducted using electrochemical impedance spectroscopy and chronopotentiometry methods. Electrical Capacitance and electrical resistance measurements were made on the membranes in various pH electrolyte solutions. The theoretical calculations were based on previously derived two theoretical models. In both models the contributions of the individual lipid molecule forms to the membrane electrical parameters were assumed to be additive. (C) 2016 Elsevier B.V. All rights reserved.