In this research, the manufacturing of highly sensitive and effective electrochemical sensor is presented. It is based on silver nanoparticles/poly(3-methylthiophene) (Ag-NPs/P3MT) nanocomposite for the detection of galantamine hydrobromide (Gal). In this process, Ag-NPs/P3MT modified electrode was fabricated by electropolymerization of 3-methylthiophene (3MT) in the presence of silver nitrate on the surface of the glassy carbon electrode (GCE). Scanning electron microscopy (SEM), field emission scanning electron microscopy (FE-SEM) and energy dispersive X-ray (EDX) analysis were employed to characterize the constructed sensor. Compared with unmodified and P3MT modified electrodes, the Ag-NPs/P3MT modified electrode demonstrated superb electrochemical catalytic activity and significantly enhanced the oxidation peak current of Gal. The experimental parameters, such as supporting electrolyte and its pH, the number of cycle's for the electro-polymerization and the scan rate for the sensor preparation were optimized. Under the optimal conditions, the proposed electrode exhibited excellent sensing performance for detecting of Gal using differential pulse voltammetry over the concentration range of 1.0 to 700 mu M and a detection limit of 0.18 mu M. Finally, the excellent results showed that the modified electrode can be successfully applied to quantitatively measure of Gal in human cerebrospinal fluid (CSF), Narcissus and pharmaceutical real samples with satisfactory consequences. (C) 2016 Elsevier B.V. All rights reserved.