In the recent years, zeolite T has been demonstrated as a potential materials for adsorption, catalysis, pervaporation as well as gaseous separation processes. However, the reported literature on the application of zeolite T as inorganic filler for the fabrication of mixed matrix membranes (MMMs) in CO2/CH4 separation is not available. Therefore, in the present work, different loadings of zeolite T particles are embedded in 6FDA-durene polyimide. The morphology and structural properties of the resultant membranes were investigated using different analytical tools and the performance of the membranes in CO2 and CH4 gases separation were tested. The results showed that CO2 permeability of 843.6 Barrer and CO2/CH4 ideal selectivity of 19.1 were obtained using 1 wt% loaded zeolite T/6FDA-durene MMM, which were 80% and 172% higher than the CO2 permeability and CO2/CH4 ideal selectivity attained using pristine 6FDA-durene. Besides, the membrane showed improvement in CO2 plasticization resistant up to 20 bar as compared to pristine 6FDA-durene membrane, which showed only 5 bar. Overall, zeolite T/6FDA-durene mixed matrix membranes fabricated in this work exhibited significant enhancement in CO2/CH4 separation, which makes them an attractive candidate for industrial gas separation especially for natural gas purification.