A method for controlling electrostatic charge on particles with positive ions and/or electrons generated by an atmospheric pressure plasma jet was studied experimentally. The atmospheric pressure plasma jet was generated by applying AC voltage and DC bias voltage to a cylindrical electrode in a quartz glass tube with helium gas flow. By changing the value of the DC bias voltage, positive ions and/or electrons could be extracted from the plasma. Two experiments, one in which particles were allowed to fall in the plasma jet and the other in which particles were irradiated with the plasma jet, were performed, and comparison of their results showed that both methods allowed the particles to acquire positive ions and/or electrons because of the electric field caused by the space charge. In addition, particle charging could be controlled by applying an external electric field. Based on these experimental results, the particle charging mechanism involved was discussed.