Highly monodispersc, dendritic, and functionalized mesoporous silica nan6spheres (MSNs) with sub-200 nm size were synthesized in a one-pot sol gel reaction, by a dual-templating micelle system consisting of a partially fluorinated short chain anionic fluorocarbon surfactant and cetyltrimethylammonium bromide. This-kind of anionic fluorocarbon surfactant works simultaneously as a swelling agent to enlarge the pore of the MSNs,, an ion-pair agent to-the structure-direeting, silane in the preparation of amine-functionalized MSNs,, and a surface tension reducing agent to make the system thermodynamically more stable for producing more uniform MSNs. The particle size and the morphology Of the resultant MSNs can be fine-tuned by changing the- amount of the fluorocarbon surfactant added and the ratio of the ftinctional group containing organosilane to tetraethoxysilane. Subsequently, the as-prepared MSNs were used as base materials,for the preparation of drug delivery nanomaterials through the surface grafting of a pH-sensitive drug-conjugated polymer and-fluorescent nanomaterials through the embedding of europium(III) complex or the immobilization Of large molecule fluorescein isothiocyanate-bovine serum albumin