Polyhexamethylene Biguanide로 기능화된 하이퍼브랜치 고분자의 항균 작용 향상

Xu Meng; Bin Wang; Liping Liang; Bailing Liu

Polymer(Korea), Vol.41, No.2, 212-220, March, 2017

DOI10.7317/pk.2017.41.2.212 Export Citation

Polyhexamethylene Biguanide로 기능화된 하이퍼브랜치 고분자의 항균 작용 향상

Polyhexamethylene Biguanide Functionalized Hyperbranched Polymers Enhance Their Antimicrobial Activities

Xu Meng^{1, 2}, Bin Wang², Liping Liang^{3, †}, and Bailing Liu^{2, †}

¹College of Textile and Garment, Shaoxing University, Shaoxing, 312000, China, Korea
²Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu, 610041, China
³College of Life Science, Shaoxing University, Shaoxing, 312000, China

E-mail:,

Hyperbranched antibacterials show much more potential performances than their small molecules due to unique structure. Polyhexamethylene biguanide (PHMB) functionalized hyperbranched poly(amide-PHMB)s (DPBs) were prepared and their antibacterial properties were investigated using several techniques. The results showed that Gram-positive organisms were more susceptible than Gram-negative bacteria for DPBs, N1, N3, N5-tris(2-aminoethyl)benzene-1,3,5-tricarboxamide (DT) and PHMB. DPBs had better comprehensive properties than DT and PHMB, and the best one was DPB1. SEM and AFM were utilized to investigate the interactions between DPB1 and bacterial membranes of E. coli. The mode of action of DPB1 was revealed by the morphological images, which was consistent with bactericidal mechanism of cationic antibacterial agents.

Keywords:hyperbranched polymer;polyhexamethylene biguanide;antimicrobial activity;antimicrobial mechanism

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[2] Chen C, Cooper SL, Tan NCB, Chem. Commun., 1, 1585 (1999)

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[7] Jikei M, Kakimoto M, Prog. Polym. Sci, 26, 1233 (2001)

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[10] Chen CZS, Cooper SL, Adv. Mater., 12(11), 843 (2000)

[11] Welk A, Splieth C, Schmidt-Martens G, J. Clin. Periodontol., 32, 499 (2005)

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[13] Gerli S, Bavetta F, Eur. Rev. Med. Pharmacol. Sci., 16, 1994 (2012)

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[16] Kusnetsov J, Tulkki A, Ahonen H, Martikainen P, J. Appl. Microbiol., 82, 763 (1997)

[17] Rebong RA, Santaella RM, Goldhagen BE, Invest. Ophth. Vis. Sci., 52, 7309 (2011)

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[22] Rosin M, Welk A, Kocher T, Majic-Todt A, Kramer A, Pitten F, J. Clin. Periodontol., 29, 392 (2002)

[23] Allen MJ, White GF, Morby AP, Microbiology, 152, 989 (2006)

[24] Jiang Z, Wang B, Che H, Liu B, J. Macromol. Sci. A, 49, 952 (2012)

[25] Qian L, Xiao H, Zhao G, He B, ACS Appl. Mater. Inter., 3, 1895 (2011)

[26] Qian LY, Guan Y, He BH, Xiao HN, Polymer, 49(10), 2471 (2008)

[27] Andrews JM, Chemother., 48, 5 (2001)

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[29] Heifets L, Am. Rev. Respir. Dis., 137, 1217 (1988)

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[31] Vandenbossche I, Vaneechoutte M, Vandevenne M, De Baere T, Verschraegen G, J. Clin. Microbiol., 40, 918 (2002)

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[33] Eaton P, Fernandes JC, Pereira E, Pintado ME, Malcata FX, Ultramicroscopy, 108, 1128 (2008)