We report the results of our studies on optical and thermal properties of binary mixture of compounds, viz. Berberine (BBE) and glacial acetic acid (GAA). The mixture exhibits a very interesting coexistent nematic biphase (N+I) and nematic (N) phase for different concentrations of BBE sequentially when the specimen is cooled from its isotropic phase. Temperature variations in optical density and ultrasonic measurements have been discussed.