(E)-N-(4-isopropylbenzylidene)thiophene-2-carbohydrazide (L) was prepared and characterized using elemental analysis, UV-visible, IR, H-1 and C-13 NMR, mass spectra. The crystal structure of L was determined by single-crystal X-ray diffraction method. The compound L crystallizes in monoclinic space group P2(1)/n with a = 16.676(5) angstrom, b = 5.475(5) angstrom, c = 17.022(5) angstrom, and Z = 4. Antimicrobial and antifungal activity was assayed against four bacterial and four fungal microorganisms. The antioxidant behavior of L was also investigated. [GRAPHICS] .