Protein-based therapeutics, in particular monoclonal antibodies (mAbs), are in high demand for treating a variety of conditions. Here, membrane chromatography for protein purifications is reviewed. In particular, current status and development for various capture and polishing steps including protein A, ion exchange and hydrophobic interaction chromatography (HIC) are discussed. In addition, new developments in ligand design and membrane functionalization for membrane chromatography are discussed. In-depth discussions on polymeric ligands with UV-initiated polymerization or atom transfer radical polymerization (ATRP) are included. Finally in silico simulations to help design new ligands and elucidate the binding interactions are also briefly reviewed.