In this work, laser micropolishing (L mu P) was employed to reduce the surface roughness and waviness of cold-rolled AISI 304 stainless steel sheets. A pulsed fibre laser operating in the ns regime was used and the influence of laser parameters in a N-2-controlled atmospheres was evaluated. In the optimal conditions, the surface remelting induced by the process allowed to reduce the surface roughness by closing cracks and defects formed during the rolling process. Other conditions that did not improve the surface quality were analysed for defect typology. Moreover, laser treatments allowed the production of more hydrophobic surfaces, and no surface chemistry modification was identified. Surface cleanability was investigated with Escherichia coli (E.coli), evaluating the number of residual bacteria adhering to the substrate after a washing procedure. These results showed that L mu P is a suitable way to lower the average surface roughness by about 58% and average surface waviness by approximately 38%. The L mu P process proved to be effective on the bacteria cleanability as approximately five times fewer bacteria remained on the surfaces treated with the optimized L mu P parameters compared to the untreated surfaces. (C) 2017 Elsevier B.V. All rights reserved.