As part of a concept for a continuous biorefining process, a fractionation procedure for lignocellulosic biomass based on flow-through organosolv lignin solubilisation has been developed and tested. Lignin is removed from the biomass in a continuous flow-through system, producing a dissolved lignin fraction and a residual fibre fraction rich in cellulose. A solvent system comprising ethanol, water and sulfuric or formic acid as catalyst is tested over a 413-468 K temperature range. Process conditions that are suitable for fractionation of softwood from Norway spruce are determined using an experimental design. The best conditions give yields up to 86% of the initially determined lignin mass, leaving a clean fibre residue that corresponds to 67% of the initial cellulose mass. The produced lignin fraction can be converted by formic acid assisted solvolysis to a liquid comprising mostly methoxy-phenols at nearly 90% mass yield. The results show that the continuous flow fractionation of lignocellulosic biomass is a promising route for biorefinery processing. (C) 2017 Elsevier Ltd. All rights reserved.