A synthetic hormone, 17 alpha-ethynylestradiol (EE2), has received much attention due to its widespread occurrence and adverse effects on aquatic animals. The removal of EE2 from water has been very challenging mostly because of its low concentration in environmental waters. Here we report a simple and novel approach for concentrating EE2 through adsorption and elution using microporous polyethersulfone (PES) hollow fiber membrane in a cross-flow filtration system, which can be easily scalable for practical applications. Our results showed that microporous PES hollow fiber membrane has a high EE2 adsorption capability (similar to 100% from water containing 20 mu g/L, of EE2) and the adsorbed EE2 could be eluted using a small Volume of 10% ethanol solution to achieve a recovery efficiency of 75.9 8.5%. We further demonstrated that the PES hollow fiber membrane maintained 100% removal of EE2 throughout five cycles of adsorption and elution. Our results from molecular simulation suggest that the strong affinity between PES and EE2 is mainly attributed to the pi-pi interaction and the hydrogen bonding. This proof-of-concept demonstrates for the first time that PES hollow fiber membrane system can remove EE2 at low concentrations, and elute EE2 solutions at a much higher concentration over multiple cycles in a sustainable and environmentally-benign condition.