Ovalbumin (OVA) is the most abundant protein in egg whites, which are often by-products of food manufacturing processes that only use the egg yolks. We previously reported that the OVA secretory signal peptide (pN1-22, acetyl-GSIGAASMEFCFDVFKELKVHH) promoted the aggregation of some proteins including OVA and enhanced the stiffness of some hydrogels including collagen gels. In this study, collagen gels containing pN1-22 were prepared and used as a cell scaffold. pN1-22 affected the gel network structure and the hydrophobicity. Mouse embryonic fibroblasts (NIH3T3 cells) were cultured on pN1-22-containing collagen gels. The morphology and the number of the adhered cells were affected by adding OVA secretory signal peptides to the collagen gel.