The present work reports the crude cellulase production using Saccharomyces cerevisiae strain MTCC 4779 and utilization of the crude enzyme in the hydrolysis of Musa cavendish pseudostem. The lignocellulosic-rich pseudostem was enzymatically hydrolyzed to glucose, which was further utilized by S. cerevisiae to produce bio-ethanol, and the distilled product had a concentration of 0.34 g/L. Conclusively, the presence of bio-ethanol was confirmed by qualitative analysis using gas chromatography coupled with flame ionization detection (GC-FID).