Hydrogen production from biomass in particular bio-oils appears interesting as bio-oils is easy to transport and storage with high conversion towards hydrogen. Phenol as presentation of lignin-derived bio-oils was chosen in this paper and was studied under aqueous phase reforming (APR) reaction using Nickel-based catalysts with ZSM-5 as support. The catalysts were synthesized by incipient wetness impregnation, and their physical and chemical properties were characterized by XRD, NH3-TPD, H-2-TPR, SEM, TEM and N-2 adsorption-desorption. Ni/ZSM-5 was studied with different Si/Al molar ratio and different Ni content on APR of phenol. The reactant concentration, reaction pressure and temperature were also evaluated. Ni/ZSM-5 with Si/Al molar ratio of 25 and nickel content of 16% exhibited the highest catalytic activity. Hydrogen production were maximized over the temperature of 240 degrees C, reaction pressure of 4 MPa and the phenol concentration of 0.2 mol/L. (C) 2016 Published by Elsevier Ltd on behalf of Hydrogen Energy Publications LLC.