AimsTo determine the presence of Clostridium difficile on fattening pig farms in north-eastern Spain. Methods and ResultsTwenty-seven farms were sampled. Pools of pig faecal samples (n=210), samples of intestinal content from common farm pest species (n=95) and environment-related samples (n=93) were collected. Isolates were tested for toxin genes of C. difficile, and typed by PCR-ribotyping and toxinotyping. The minimal inhibitory concentrations of six antimicrobial agents were determined using Etest. Thirty-four isolates were obtained from 12 farms, and 30 (882%) had toxin genes. Seven ribotypes were identified. Ribotype 078 and its variant 126 were predominant (529%). The same ribotypes were isolated from different animal species on the same farm. None of the isolates were resistant to metronidazole or vancomycin. ConclusionsClostridium difficile was common within the pig farm environment. Most of the positive samples came from pest species or were pest-related environmental samples. Significance and Impact of the StudyPest species were colonized with toxigenic and antimicrobial-resistant C. difficile strains of the same ribotypes that are found in humans and pigs. Rodents and pigeons may transmit toxigenic and antimicrobial-resistant C. difficile strains that are of the same ribotypes as those occuring in humans.