AimsThe aim of this work was to evaluate the efficacy and safety of Lippia origanoides essential oil as a preservative in industrial products. Methods and ResultsThe composition, antimicrobial activity, mutagenic and toxic potential of L. origanoides were determined. Then, the effect of essential oil as a preservative in food, cosmetics and pharmaceutical products was evaluated. The essential oil of L. origanoides consisted mainly of oxygenated monoterpenes (3813%); 2628% corresponded to the compound carvacrol. At concentrations ranging from 0312 to 125lml(-1) and in association with polysorbate 80, the essential oil of L. origanoides inhibited the growth of all the tested micro-organisms. The medium lethal dose in mice was 35gkg(-1), which categorizes it as nontoxic according to the European Union criteria, and negative results in the Ames test indicated that this oil was not mutagenic. In combination with polysorbate 80, the essential oil exerted preservative action on orange juice, cosmetic and pharmaceutical compositions, especially in the case of aqueous-based products. ConclusionsLippia origanoides essential oil is an effective and safe preservative for orange juice, pharmaceutical and cosmetic products. Significance and Impact of the StudyThis study allowed for the complete understanding of the antimicrobial action and toxicological potential of L.origanoides essential oil. These results facilitate the development of a preservative system based on L. origanoides essential oil.