We described the synthesis of biscuit-like copper oxide (CuO) by the precipitation cum thermal annealing process. The biscuit-like CuO microstructures were successfully obtained by template free synthesis process. Thereby, the oxalic acid was used as the shape forming agent. Herein, the role of the sonic wave was quite important to controlling the shape. The CuO microstructures were characterized by the X-ray diffraction pattern, scanning electron microscope and energy dispersive X-ray analysis. The as prepared CuO was used to fabricate the disposable sensor electrode using screen printed carbon electrode (SPCE). The CuO modified SPCE was successfully determined the glucose with the linear concentration ranging from 0.0005 to 4.03 mM and the lowest detection limit of 0.1 mu M. The biscuit-like CuO microstructures based glucose sensor displayed appreciable analytical performance than the other CuO nanostructures. Moreover, the disposable CuO/SPCE was applied to determine the glucose in human blood serum, saliva and urine samples. The developed glucose sensor attained good recoveries in real sample analysis, hence, it is applicable for the commercial applications. (C) 2017 Elsevier Inc. All rights reserved.