In this work, Cu(II)-exchanged clinoptilolite nanoparticles (Cu(II)-NCL) were prepared and characterized by FTIR, BET, XRD and TEM techniques. The obtained Cu(II)-NCL was then used for the modification of carbon paste electrode (CPE). The resulted Cu(II)-NCL/CPE was finally used for the voltammetric determination of amoxicillin (AMX). The best voltammetric response was obtained by the electrode containing 20% of the modifier in 0.05 mol L-1 NaCl at pHs 2.2 and 7.2. The electrode showed a linear response in the concentration range of 4.0 x 10(-8)-1.0 x 10(-4) mol L-1 AMX with a detection limit of 2.0 x 10(-8) mol L-1 in square wave voltammetry. The electrode showed good repeatability, reproducibility, long life time confirmed by statistical tests. The electrode had also good selectivity together with good applicability in determination of AMX in urine and pharmaceutical tablets as real samples. (C) 2017 Elsevier Inc. All rights reserved.