Inconsistent results between polycyclic aromatic hydrocarbons (PAHs) exposure and adverse male reproductive health have been reported in humans. To assess whether PAH exposure is associated with declined sperm function. Ten monohydroxylated PAHs (OH-PAHs) metabolites Were analyzed in repeated urine samples from an infertility clinic. We used multivariable linear models to estimate the associations of urinary OH-PAH metabolites with sperm DNA damage (n = 405) and spermatozoa apoptosis (n = 366). The shapes of dose-dependent associations of exposure measurements with outcomes were further evaluated by restricted cubic splines. Multiple comparisons were adjusted by false discovery rate (FDR). We found that urinary 9-hydroxyfluorene (9-OHFIu) was associated with increased tail length and comet length (p for trend = 0.05 and 0.01, respectively), and that urinary 9-hydroxyphenanthrene (9-0HPh) was associated with decreased percentage of Annexin V-/PI- spermatozoa (p for trend = 0.04). Also, suggestive associations of urinary 9-OHPh and Sigma OHFIu with increased comet length, and urinary 9-OHFIu and 2-OHPh with decreased percentage of Annexin V-/PI- spermatozoa were observed (all p for trends <0.10). Further, these dose-dependent associations were confirmed in restricted cubic splines. Our results suggest that environmental exposure to fluorene and phenanthrene are associated with declined sperm function. (C) 2017 Elsevier B.V. All rights reserved.