Ibuprofen (IBU) is one of the most widely used and frequently detected human pharmaceuticals in aquatic environment. However, the toxicity of IBU on diatom and its fate remain still unkown. In the present study, the toxicity of IBU on the diatom was evaluated by the algal growth rate, the chlorophyll-a and carotenoids contents. The degradation of IBU including in particular the potential for the formation of incomplete degradation products was also explored. Biochemical characteristics of Navicula sp. were significantly inhibited at IBU concentrations up to 50 mg L-1 after 10 days of exposure. The degradation of IBU was retarded by Navicula sp. at low concentration (1 mg L-1), with t(1/2) being extended from 9.6 +/- 1.8 d to 12.0 +/- 1.5 d, indicating that Navicula sp. could prolong the exposure time of IBU. A total of 8 metabolites were identified by LC-MS/MS and the degradation pathway of IBU in Navicula sp, was proposed. Hydroxylation, acylation, demethylation, and glucuronidation contributed to IBU transformative reactions in diatom cells. These findings indicate that the presence of diatom Navicula sp. could hinder degradation of IBU, and IBU and/or its metabolites may pose high risks on aquatic ecosystem in natural waters. (C) 2017 Elsevier B.V. All rights reserved.