The presence of migrated petroleum in outcropping rocks on Spitsbergen (Svalbard archipelago) has been known for several decades but the petroleum has not been evaluated by modern geochemical methods. This paper presents detailed organic geochemical observations on bitumen in outcrop samples from central and eastern Spitsbergen. The samples comprise sandstones from the Lower Cretaceous Carolinefjellet Formation, the Upper Triassic -Middle Jurassic Wilhelmoya Subgroup and the Upper Triassic De Geerdalen Formation; a limestone from the De Geerdalen Formation; and carbonates from the Middle Jurassic -Lower Cretaceous Agardhfjellet Formation. In addition a palaeo-seepage oil was sampled from a vug in the Middle Triassic Botneheia Formation. This data is integrated with the results of analyses of C-1 - C-4 hydrocarbon fluid inclusions trapped in quartz and calcite cements in these samples. Organic geochemical data suggest that the petroleum present in the samples analysed can be divided into two compositional groups (Group I and Group II). Group I petroleums have distinctive biomarker characteristics including Pr/ Ph ratios of about 1.3-1.5, high tricyclic terpanes relative to pentacyclic terpanes, and relatively high methyl-dibenzothiophenes compared to methyl-phenanthrenes. By contrast Group II petroleums have low tricyclic terpanes relative to pentacyclic terpanes and low methyl-dibenzothiophenes compared to methyl-phenanthrenes, and most Pr/Ph ratios range from 1.90 to 2.57. The petroleum in both groups was derived from marine shale source rocks deposited in proximal to open marine settings. Group I petroleums, present in the sandstones of the Wilhelmoya Subgroup and the De Geerdalen Formation and as a palaeo-seepage oil in the vug in the Botneheia Formation, are likely to have been sourced from the Middle Triassic Botneheia Formation. Group II petroleums,