Inkjet-based multilayered growth factor-releasing nanofilms for enhancing proliferation of mesenchymal stem cells in vitro

Moonhyun Choi; Daheui Choi; Uiyoung Han; Jinkee Hong

Journal of Industrial and Engineering Chemistry, Vol.50, 36-40, June, 2017

DOI10.1016/j.jiec.2017.02.014 Export Citation

Inkjet-based multilayered growth factor-releasing nanofilms for enhancing proliferation of mesenchymal stem cells in vitro

Moonhyun Choi, Daheui Choi, Uiyoung Han, and Jinkee Hong^†

School of Chemical Engineering and Material Science, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea

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We report the preparation and characterization of inkjet-based basic fibroblast growth factor (bFGF)- containing nanofilms on a flexible PET substrate. bFGF and heparin (HEP) were assembled by inkjet-based layer-by-layer (LbL) assembly driven by electrostatic interactions. The bFGF/HEP nano-assembly surface coatings were formed via alternating printing adsorption of positively charged bFGF and negatively charged HEP; the process was monitored by UV.vis spectroscopy and quartz crystal microbalance. The bFGF release profile could be controlled by altering the number of layers of printed LbL films. Mesenchymal stem cells, which are capable of extended proliferation in vitro, require a continuous supply of bFGF for proliferation. However, enhancing mesenchymal stem cell proliferation by continuous supplying bFGF is difficult, even with medium replacement, because of the instability of bFGF. Here, we established a novel system for releasing bioactive bFGF from a modified surface by using an inkjet-based nanofilm fabrication method.

Keywords:Ink-jet printing;Nanofilms;Layer-by-layer (LbL) assembly;Basic;fibroblast growth factor;Mesenchymal stem cells (MSCs)

[References]

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Nardi NB, da Silva Meirelles L, Stem Cells, Springer, 2008 pp. 249.
Heino TJ, Hentunen TA, Curr. Stem Cell Res. Ther., 3, 131 (2008)
Fukuda K, Artif. Organs, 25, 187 (2001)
Nazempour A, Van Wie BJ, Ann. Biomed. Eng., 44, 1325 (2016)
Scott MA, Nguyen VT, Levi B, James AW, Stem Cells Dev., 20, 1793 (2011)
Bara JJ, Richards RG, Alini M, Stoddart MJ, Stem Cells, 32, 1713 (2014)
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Tasso R, Augello A, Boccardo S, Salvi S, Carida M, Postiglione F, Fais F, Truini M, Cancedda R, Pennesi G, Tissue Eng., 15, 2203 (2009)
Tasso R, Fais F, Reverberi D, Tortelli F, Cancedda R, Biomaterials, 31, 2121 (2010)
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Caplan AI, Correa D, Cell Stem Cell, 9, 11 (2011)
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Levenstein ME, Ludwig TE, Xu RH, Llanas RA, VanDenHeuvel-Kramer K, Manning D, Thomson JA, Stem Cells, 24, 568 (2006)
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Tanihara M, Suzuki Y, Yamamoto E, Noguchi A, Mizushima Y, J. Biomed. Mater. Res., 56, 216 (2001)
Cai S, Liu Y, Shu XZ, Prestwich GD, Biomaterials, 26, 6054 (2005)
Nguyen TH, Kim SH, Decker CG, Wong DY, Loo JA, Maynard HD, Nat. Chem., 8, 221 (2013)
Choi SM, Ryu HA, Lee KM, Kim HJ, Park IK, Cho WJ, Shin HC, Choi WJ, Lee JW, Biomater. Res., 20, 1 (2016)
Fannon M, Forsten KE, Nugent MA, Biochemistry, 39, 1434 (2000)
Wang YJ, Shahrokh Z, Vemuri S, Eberlein G, Beylin I, Busch M, Formulation, Characterization, and Stability of Protein Drugs: Case Histories, Springer, 2002 pp. 141.
Chen G, Gulbranson DR, Yu P, Hou Z, Thomson JA, Stem Cells, 30, 623 (2012)
Decher G, Science, 277(5330), 1232 (1997)
Richardson JJ, Bjornmalm M, Caruso F, Science, 348, aaa249 (2015)
Cassier T, Lowack K, Decher G, Supramol. Sci., 5, 309 (1998)
Zhang HY, Fu Y, Wang D, Wang LY, Wang ZQ, Zhang X, Langmuir, 19(20), 8497 (2003)
Wood KC, Chuang HF, Batten RD, Lynn DM, Hammond PT, Proc. Natl. Acad. Sci. U. S. A., 103, 10207 (2006)
Hong J, Kim BS, Char K, Hammond PT, Biomacromolecules, 12(8), 2975 (2011)
Macdonald ML, Rodriguez NM, Shah NJ, Hammond PT, Biomacromolecules, 11(8), 2053 (2010)
Shah NJ, Hong J, Hyder MN, Hammond PT, Adv. Mater., 24(11), 1445 (2012)
Cho Y, Lee JB, Hong J, Chem. Eng. J., 221, 32 (2012)
Cho Y, Lee JB, J. Hong, Sci. Rep., 4 (2014)
Tao H, Marelli B, Yang MM, An B, Onses MS, Rogers JA, Kaplan DL, Omenetto FG, Adv. Mater., 27(29), 4273 (2015)
Guo YL, Ono Y, Nagao Y, Langmuir, 31(37), 10137 (2015)
Gekko K, Timasheff SN, Biochemistry, 20, 4667 (1981)
Farnum M, Zukoski C, Biophys. J., 76, 2716 (1999)
Rariy RV, Klibanov AM, Proc. Natl. Acad. Sci. U. S. A., 94, 13520 (1997)
Vagenende V, Yap MG, Trout BL, Biochemistry, 48, 11084 (2009)
Saksela O, Moscatelli D, Sommer A, Rifkin DB, J. Cell Biol., 107, 743 (1988)
Arakawa T, Wen J, Philo JS, Arch. Biochem. Biophys., 308, 267 (1994)
Faham S, Hileman RE, Fromm JR, Linhardt RJ, Rees DC, Science, 271(5252), 1116 (1996)
Schlessinger J, Plotnikov AN, Ibrahimi OA, Eliseenkova AV, Yeh BK, Yayon A, Linhardt RJ, Mohammadi M, Mol. Cell. Biochem., 6, 743 (2000)
Picart C, Lavalle P, Hubert P, Cuisinier FJG, Decher G, Schaaf P, Voegel JC, Langmuir, 17(23), 7414 (2001)
Shiratori SS, Yamada M, Polym. Adv. Technol., 11, 810 (2000)
Fukao N, Kyung KH, Fujimoto K, Shiratori S, Macromolecules, 44(8), 2964 (2011)

[Referenced By]

[1] Prockop DJ, Science, 276(5309), 71 (1997)

[2] Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR, Science, 284, 143 (1999)

[3] Nardi NB, da Silva Meirelles L, Stem Cells, Springer, 2008 pp. 249.

[4] Heino TJ, Hentunen TA, Curr. Stem Cell Res. Ther., 3, 131 (2008)

[5] Fukuda K, Artif. Organs, 25, 187 (2001)

[6] Nazempour A, Van Wie BJ, Ann. Biomed. Eng., 44, 1325 (2016)

[7] Scott MA, Nguyen VT, Levi B, James AW, Stem Cells Dev., 20, 1793 (2011)

[8] Bara JJ, Richards RG, Alini M, Stoddart MJ, Stem Cells, 32, 1713 (2014)

[9] Picinich SC, Mishra PJ, Mishra PJ, Glod J, Banerjee D, Expert Opin. Biol. Ther., 7, 965 (2007)

[10] Tasso R, Augello A, Boccardo S, Salvi S, Carida M, Postiglione F, Fais F, Truini M, Cancedda R, Pennesi G, Tissue Eng., 15, 2203 (2009)

[11] Tasso R, Fais F, Reverberi D, Tortelli F, Cancedda R, Biomaterials, 31, 2121 (2010)

[12] Tolar J, Le Blanc K, Keating A, Blazar BR, Stem Cells, 28, 1446 (2010)

[13] Yagi H, Soto-Gutierrez A, Parekkadan B, Kitagawa Y, Tompkins RG, Kobayashi N, Yarmush ML, Cell Transplant., 19, 667 (2010)

[14] Caplan AI, Correa D, Cell Stem Cell, 9, 11 (2011)

[15] Le Blanc K, Pittenger M, Cytotherapy, 7, 36 (2005)

[16] Kang YJ, Jeon ES, Song HY, Woo JS, Jung JS, Kim YK, Kim JH, J. Cell. Biochem., 95, 1135 (2005)

[17] Xin H, Li Y, Chen X, Chopp M, J. Neurosci. Res., 83, 1485 (2006)

[18] Wang L, Huang Y, Pan K, Jiang X, Liu C, Ann. Biomed. Eng., 38, 77 (2010)

[19] Yuan S, Pan Q, Fu CJ, Bi Z, Indian J. Med. Res., 138, 104 (2013)

[20] Ornitz DM, Xu J, Colvin JS, McEwen DG, MacArthur CA, Coulier F, Gao G, Goldfarb M, J. Biol. Chem., 271, 15292 (1996)

[21] Hori Y, Inoue S, Hirano Y, Tabata Y, Tissue Eng., 10, 995 (2004)

[22] Levenstein ME, Ludwig TE, Xu RH, Llanas RA, VanDenHeuvel-Kramer K, Manning D, Thomson JA, Stem Cells, 24, 568 (2006)

[23] Furue MK, Na J, Jackson JP, Okamoto T, Jones M, Baker D, Hata RI, Moore HD, Sato JD, Andrews PW, Proc. Natl. Acad. Sci. U. S. A., 105, 13409 (2008)

[24] Shiba T, Nishimura D, Kawazoe Y, Onodera Y, Tsutsumi K, Nakamura R, Ohshiro M, J. Biol. Chem., 278, 26788 (2003)

[25] Beenken A, Mohammadi M, Nat. Rev. Drug Discov., 8, 235 (2009)

[26] Tanihara M, Suzuki Y, Yamamoto E, Noguchi A, Mizushima Y, J. Biomed. Mater. Res., 56, 216 (2001)

[27] Cai S, Liu Y, Shu XZ, Prestwich GD, Biomaterials, 26, 6054 (2005)

[28] Nguyen TH, Kim SH, Decker CG, Wong DY, Loo JA, Maynard HD, Nat. Chem., 8, 221 (2013)

[29] Choi SM, Ryu HA, Lee KM, Kim HJ, Park IK, Cho WJ, Shin HC, Choi WJ, Lee JW, Biomater. Res., 20, 1 (2016)

[30] Fannon M, Forsten KE, Nugent MA, Biochemistry, 39, 1434 (2000)

[31] Wang YJ, Shahrokh Z, Vemuri S, Eberlein G, Beylin I, Busch M, Formulation, Characterization, and Stability of Protein Drugs: Case Histories, Springer, 2002 pp. 141.

[32] Chen G, Gulbranson DR, Yu P, Hou Z, Thomson JA, Stem Cells, 30, 623 (2012)

[33] Decher G, Science, 277(5330), 1232 (1997)

[34] Richardson JJ, Bjornmalm M, Caruso F, Science, 348, aaa249 (2015)

[35] Cassier T, Lowack K, Decher G, Supramol. Sci., 5, 309 (1998)

[36] Zhang HY, Fu Y, Wang D, Wang LY, Wang ZQ, Zhang X, Langmuir, 19(20), 8497 (2003)

[37] Wood KC, Chuang HF, Batten RD, Lynn DM, Hammond PT, Proc. Natl. Acad. Sci. U. S. A., 103, 10207 (2006)

[38] Hong J, Kim BS, Char K, Hammond PT, Biomacromolecules, 12(8), 2975 (2011)

[39] Macdonald ML, Rodriguez NM, Shah NJ, Hammond PT, Biomacromolecules, 11(8), 2053 (2010)

[40] Shah NJ, Hong J, Hyder MN, Hammond PT, Adv. Mater., 24(11), 1445 (2012)

[41] Cho Y, Lee JB, Hong J, Chem. Eng. J., 221, 32 (2012)

[42] Cho Y, Lee JB, J. Hong, Sci. Rep., 4 (2014)

[43] Tao H, Marelli B, Yang MM, An B, Onses MS, Rogers JA, Kaplan DL, Omenetto FG, Adv. Mater., 27(29), 4273 (2015)

[44] Guo YL, Ono Y, Nagao Y, Langmuir, 31(37), 10137 (2015)

[45] Gekko K, Timasheff SN, Biochemistry, 20, 4667 (1981)

[46] Farnum M, Zukoski C, Biophys. J., 76, 2716 (1999)

[47] Rariy RV, Klibanov AM, Proc. Natl. Acad. Sci. U. S. A., 94, 13520 (1997)

[48] Vagenende V, Yap MG, Trout BL, Biochemistry, 48, 11084 (2009)

[49] Saksela O, Moscatelli D, Sommer A, Rifkin DB, J. Cell Biol., 107, 743 (1988)

[50] Arakawa T, Wen J, Philo JS, Arch. Biochem. Biophys., 308, 267 (1994)

[51] Faham S, Hileman RE, Fromm JR, Linhardt RJ, Rees DC, Science, 271(5252), 1116 (1996)

[52] Schlessinger J, Plotnikov AN, Ibrahimi OA, Eliseenkova AV, Yeh BK, Yayon A, Linhardt RJ, Mohammadi M, Mol. Cell. Biochem., 6, 743 (2000)

[53] Picart C, Lavalle P, Hubert P, Cuisinier FJG, Decher G, Schaaf P, Voegel JC, Langmuir, 17(23), 7414 (2001)

[54] Shiratori SS, Yamada M, Polym. Adv. Technol., 11, 810 (2000)

[55] Fukao N, Kyung KH, Fujimoto K, Shiratori S, Macromolecules, 44(8), 2964 (2011)