epsilon-Poly-L-lysine (s-PL) is one of the few homopoly(amino-acid)s occurring in nature. epsilon-PL, which possesses multiple amino groups, is highly soluble in water, where it forms the antimicrobial polycationic chain (PLn+). Although the high water-solubility is advantageous for the use of epsilon-PL as a food preservative, it has limited the applicability of epsilon-PL as a biopolymer plastic. Here, we report on the preparation and availability of a water-insoluble complex formed with PLn+ and an anionic surfactant, bis(2-ethylhexyl) sulfosuccinate (BEHS-, is also commercialized as AOT) anion. The PLn+/BEHS--complex, which is soluble in organic solvents, was successfully used as a coating material for a cellulose acetate membrane to create a water-resistant antimicrobial membrane. In addition, the thermoplastic PLn+/BEHS--complex was able to be uniformly mixed with polypropylene by heating, resulting in materials exhibiting antimicrobial activities.