Application of pre-aeration (AS) to waste prior to feeding was evaluated on acidogenic process in a semipilot scale biosystem for the production of biobased products (biohydrogen, volatile fatty acids (VFA) and biohythane) from food waste. Oxygen assisted in pre-hydrolysis of waste along with the suppression of methanogenic activity resulting in enhanced acidogenic product formation. AS operation resulted in 97% improvement in hydrogen conversion efficiency (HCE) and 10% more VFA production than the control. Increasing the organic load (OL) of food waste in association with AS application improved the productivity. The application of AS also influenced concentration and composition of fatty acid. Highest fraction of acetic (5.3 g/l), butyric (0.7 g/l) and propionic acid (0.84 g/l) was achieved at higher OL (100 g COD/l) with good degree of acidification (DOA). AS strategy showed positive influence on biofuel (biohydrogen and biohythane) production along with the biosynthesis of short chain fatty acids functioning as a lowcost pretreatment strategy in a single stage bioprocess. (C) 2017 Elsevier Ltd. All rights reserved.