An aerobic granular sludge system has been started-up and operated at 7 degrees C temperature using cold-adapted activated sludge as inoculum. The system could form granular biomass due to batch operation allowing for just 5-3 min of biomass sedimentation. Scanning electron microscopy showed that fungi helped in the granular biomass formation in the early stages of the granule formation. The removal performance of the system was of 92-95% in BOD5, 75-80% in COD, 70-76% in total nitrogen and 50-60% in total phosphorous. The bacterial community structure from cold-adapted activated sludge changed during the operational time, leading to a final configuration dominated by Microbacteriaceae members Microbacterium and Leucobacter, which were strongly correlated to biomass settling velocity and biore-actor performance, as suggested by multivariate redundancy analyses. This experiment showed that aerobic granular sludge systems could be successfully started-up and operated, with high performance, under low operational temperatures when using cold-adapted biomass as inoculum. (C) 2017 Elsevier Ltd. All rights reserved.