Quantitative analysis of enzyme adsorption and hydrolysis were performed for sieve-based grinding corn stover (SGCS) and ultrafine grinding corn stover (UGCS)(1) with different enzyme consumptions. The UGCS presented significantly higher enzyme adsorption quantity (5.15 mg/g for UGCS, 1.33 mg/g for SGCS), higher glucose yield (49.75% for UGCS, 28.75% for SGCS) under 20 FPU/g and higher binding enzyme proportion (41.32% for UGCS, 10.64% for SGCS under 5 FPU/g) which can be attributed to the more accessible microstructure properties. The relationship between enzyme adsorption and hydrolytic production was directly proportional for SGCS (GY(1) = 21.04 x AQ(1) + 1.86 (R-2 = 0.95)) while was exponential for UGCS (GY(2) = 49.42 x (1 e(2)(0.57xAQ)) (R-2 = 0.99)),(2) indicating that overmuch enzyme consumption was not advisable for UGCS at economical aspect. (C) 2017 Elsevier Ltd. All rights reserved.