Pullulan is a polymer produced by Aureobasidium pullulans and the main bottleneck for its industrial production is the presence of melanin pigment. In this study, light-emitting diodes (LEDs) of different wavelengths were used to assist the fermentation process aiming to produce low-melanin containing pullulan by wild strain of A. pullulans LB83 with different carbon sources. Under white light using glucose-based medium, 11.75 g.L (1) of pullulan with high melanin content (45.70 UA540nm. g (1)) was obtained, this production improved in process assisted by blue LED light, that resulted in 15.77 g.L (1) of pullulan with reduced content of melanin (4.46 UA(540nm.)g (1)). By using sugarcane bagasse (SCB) hydrolysate as carbon source, similar concentration of pullulan (about 20 g.L (1)) was achieved using white and blue LED lights, with lower melanin contents in last. Use of LED light was found as a promising approach to assist biotechnological process for low-melanin containing pullulan production. (C) 2017 Elsevier Ltd. All rights reserved.