The direct asymmetric Michael addition of malonates and enones was promoted by protease from Streptomyces griseus for the first time. Yields of up to 84% with enantioselectivities of up to 98% enantiomeric excess (ee) were achieved under optimized conditions. Protease from Streptomyces griseus (SGP) was used for the first time as a biocatalyst in asymmetric Michael reaction of malonates. [GRAPHICS] .