Many of pyridine compounds are considered as typical hazardous refractory organics due to their acute toxic properties and teratogenic effects. Therefore, it is great significant to develop a separation technology with the characters of low energy consumption and environmental compatibility to efficiently remove pyridine from its wastewater. In this work, foam fractionation was used to remove pyridine from its wastewater and a novel foam fractionation column was designed for enhancing interfacial adsorption by vertical sieve tray and strengthening foam drainage by floating tongue type tray, in which bubble diameter was monitored for adjusting feeding flow rate and air flow rate in the continuous foam fractionation process. By comparing the foam properties and the adsorption properties of three anionic surfactants (sodium dodecyl sulfate, sodium alcohol ether sulfate and sodium alpha-olefin sulfonate), sodium dodecyl sulfate was chosen as the optimal collector to remove pyridine at pH 4.5. Under the optimal operating conditions, the enrichment ratio and removal percentage of pyridine could reach as high as 34.5 and 90.2%, simultaneously. Above results indicated that foam fractionation was a practicable technology to efficiently remove a hazardous material from its wastewater by designing an effective foam fractionation column. (C) 2017 Elsevier B.V. All rights reserved.