Herein, we employed single particle dark-field (DF) and differential interference contrast (DIC) microscopy to investigate the particle size dependency for large gold nanospheres (AuNSs) with average diameters of 100 nm, 300 nm, and 400 nm. Higher order plasmon resonances were observed in the single particle scattering spectra of large AuNSs (300 nm and 400 nm). Furthermore, the DF and DIC intensities were linearly increased with increasing the particle size from 100 nm to 400 nm. Therefore, the results provide useful experimental information of how the scattering and interference properties of single AuNSs are influenced by the particle size. (C) 2017 Elsevier B.V. All rights reserved.