A new method for the NO reaction rate calculation of turbulent non-premixed flames is proposed and validated. The new model acknowledges the importance of NO reaction history, it specifies a reaction time at which the NO reaction rate represents the average NO reaction rate over the history, and this reaction time is related to locale turbulent time scale. The turbulent fluctuation effect on NO reaction rate is included by considering the fluctuation of mixture fraction and temperature. Three turbulent round jet flames with different fuel compositions are used to validate the model. The temperature field of the three flames has been predicted very well by changing the model constants of the turbulence model and the turbulent combustion model, the accurate temperature field prediction provides the necessary base for the NO reaction model validation. By comparison with the experimental data, it is shown that the NO reaction model could predict the NO concentration well. (C) 2017 Elsevier Ltd. All rights reserved.