Nickel Iron Layered Double Hydroxide nano composites were electrochemically synthesized on graphene/glassy carbon electrode at a constant potential. The surface morphology and the structure of the electrodes were characterized by scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), elemental mapping analysis, X-ray diffraction (XRD) and Atomic force microscopy (AFM). This electrode was studied for glucose electro-oxidation reaction using cyclic voltammetry, chronoamperometry and electrochemical impedance spectroscopy (EIS) techniques. Results confirmed high catalytic activity, stability of the graphene/Ni-Fe LDH electrode and glucose electro oxidation reaction on this electrode is under the effect of diffusion process. Also in comparison of some previous reported methods for the glucose electro oxidation, graphene/Ni Fe LDH shows a high diffusion coefficient as an electro catalyst for glucose electro oxidation. Electrical equivalent circuits for electrodes is obtained by using the Zview software. The low electrochemical charge transfer resistance (R-ct) was obtained on the graphene/Ni Fe LDH due to the presence of Ni-Fe LDH nano composite. (C) 2017 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.