A microfluidic fuel cell (MFC) is constructed at laboratory for NaBH4 electrooxidation using varying operating conditions. The temperatures of anode and cathode were varied from 40 degrees C to 70 degrees C, and the pressure was maintained at 1 bar. The anode and cathode electrocatalyst used was Pt (40 wt. %)/High Surface Area Carbon (CHSA) with loading in the range of 0.5 mg/cm(2) to 2 mg/cm(2). The oxidant at cathode was atmospheric oxygen (21 mol % O-2). The commercial gas diffusion layer (GDL) was used as substrate at anode and air breathing cathode side. The cell voltage and current density were measured for different fuel (NaBH4) concentration, electrolyte (KOH) concentration, temperature and electrocatalyst loading at anode and cathode, respectively. The maximum open circuit voltage (OCV) of 1.079 V and power density of 24.09 mW/cm(2) at a current density of 54.97 mA/cm(2) were obtained for anode (Pt/C-HSA) and cathode (Pt/C-HSA) loading of 1 mg/cm(2) using 0.1 M NaBH4 as fuel mixed with 1 M KOH as electrolyte at a temperature of 70 degrees C. Whereas the maximum power density of 8.47 mW/cm(2) at a current density of 34.04 mA/cm(2) was obtained at the temperature of 40 degrees C. Although similar cell conditions were used, the cell performance in terms of power density is significantly enhanced (about 65%) due to increase in temperature from 40 degrees C to 70 degrees C. These results were validated using cyclic voltammetry at single electrodes under similar conditions to those of the single microfluidic fuel cell. (C) 2016 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.